Provided is a display substrate including a base substrate, an anode layer, a light-emitting layer, an electron diffusion layer, a hole block layer and a cathode layer which are sequentially stacked along a direction away from the base substrate, wherein a material of the electron diffusion layer comprises a first luminescent material, and a transport speed of holes generated by the anode layer in the electron diffusion layer is less than a transport speed of the holes in the light-emitting layer.