A display substrate has a screen area, the screen area includes at least one sensing area and a display area surrounding each sensing area, the sensing area includes a light-transmitting area and a routing area. The display substrate includes: a plurality of first gate lines, each of which includes a gate line main body part in the display area and a gate line connecting part; and a plurality of first data lines, each of which includes a data line main body part in the display area and a data line connecting part. For any one of the routing areas, the plurality of data line connecting parts are respectively in at least two metal layers insulated and spaced from each other, and/or, the plurality of gate line connecting parts are respectively arranged in at least two metal layers insulated and spaced from each other.