Various embodiments and methods related to an optical fiber alignment system are provided herein. The optical fiber alignment system includes a controller and a rotation stage having a central axis, a first end, and a second end. The central axis extends from the first end to the second end of the rotation stage. The rotation stage includes an optical fiber channel extending from the first end of the rotation stage to the second end of the rotation stage and may be operationally coupled with the controller. The rotation stage is configured to rotate about the central axis of the rotation stage. The optical fiber alignment system includes a light source positioned to emit light onto the optical fiber channel at an oblique angle from the central axis of the rotation stage. The optical fiber alignment system includes an image sensor positioned adjacent to the second end of the rotation stage.