发明申请
US20020061546A1 Assays for protein kinases using fluorescent protein substrates
审中-公开
使用荧光蛋白底物测定蛋白激酶
- 专利标题: Assays for protein kinases using fluorescent protein substrates
- 专利标题(中): 使用荧光蛋白底物测定蛋白激酶
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申请号: US09884681申请日: 2001-06-19
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公开(公告)号: US20020061546A1公开(公告)日: 2002-05-23
- 发明人: Roger Y. Tsien , Andrew B. Cubitt
- 申请人: The Regents of the University of California
- 申请人地址: null
- 专利权人: The Regents of the University of California
- 当前专利权人: The Regents of the University of California
- 当前专利权人地址: null
- 主分类号: C12Q001/48
- IPC分类号: C12Q001/48 ; C12N009/12
摘要:
This invention provides assays for protein kinase activity using fluorescent proteins engineered to include sequences that can be phosphorylated by protein kinases. The proteins exhibit different fluorescent properties in the non-phosphorylated and phosphorylated states.
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