A method for refining fluid sample data includes obtaining optical density data for a fluid sample in at least two color channels and at least one fluid component channel and determining a color-absorption function from the optical density data for the fluid sample in the at least two color channels. The method also includes calculating a portion of the optical density caused by color absorptions in each of the at least one fluid component channels, and de-coloring the optical density data in each of the at least one fluid component channels by removing the portion of the optical density data caused by color absorption.