A detection device, in particular for use in a laser scanning microscope, includes a means (2) located in a detection beam path (1) to spectrally split detection light into individual spectral components (3, 4), and further includes a deflection device (5) located downstream of the means (2) for spectral splitting to deflect the individual spectral components (3, 4) in different deflection directions onto detectors (6) assigned to the individual spectral components (3, 4). With a view to reliable separation of the individual spectral components (3, 4) deflected by deflection device (5), the detection device is built and further refined in such a way that at least one optical element (7) is arranged in the detection beam path (1) downstream of the means (2) for spectral splitting and upstream of the deflection device such that at least one spectral component (3, 4) of the light incident on the deflection device (5) is collimated in at least one spatial direction.