发明申请
- 专利标题： Ligand binding assays for vanilloid receptors
- 专利标题（中）： 香草素受体的配体结合测定
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申请号： US10922683申请日： 2004-08-19
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公开(公告)号： US20050019958A1公开(公告)日： 2005-01-27
- 发明人: Sui-Po Zhang , Ellen Codd
- 申请人： Sui-Po Zhang , Ellen Codd
- 主分类号： G01N33/543
- IPC分类号： G01N33/543 ; G01N33/53 ; G01N33/566 ; G01N33/536
摘要：
The present invention describes an assay in which pH alters RTX affinity to VR1 receptors. The RTX affinity was increased with increasing pH from 4.1 to 8.6. Both the RTX affinity and apparent number of RTX binding sites were decreased with increasing pH from 8.6 to 10.6. The high pH may be causing the cell membranes to denature. These pH conditions (pH 8.0 to pH 9.0) provide a high signal to noise ratio, give a more robust assay and require use of less experimental materials.
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