发明申请
- 专利标题： Materials and methods to produce stem cells
- 专利标题（中）： 生产干细胞的材料和方法
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申请号： US10869393申请日： 2004-06-16
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公开(公告)号： US20050032208A1公开(公告)日： 2005-02-10
- 发明人: Steve Oh , Andre Choo
- 申请人： Steve Oh , Andre Choo
- 主分类号： C12N5/0735
- IPC分类号： C12N5/0735 ; C12N15/85 ; C12Q1/68 ; C12N5/08
摘要：
A method of generating stem cells, such as human and mouse embryonic stem cells, from a stem cell culture comprising the step of perfusing the stem cell culture by flowing culture media through the stem cell culture and apparatus for generating stem cells are disclosed. Also disclosed are methods and apparatus for generating stem cells, such as human and mouse embryonic cells, by culturing the cells on a hydrophilic plastics membrane. Further disclosed is a supplemented conditioned medium for obtaining improved yields of stem cells, such as human and mouse embryonic stem cells.
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