发明申请
US20050064488A1 Method for detecting a target nucleic acid by using a detection probe capable of hybridizing with a tag sequence 审中-公开
通过使用能够与标签序列杂交的检测探针来检测靶核酸的方法

  • 专利标题: Method for detecting a target nucleic acid by using a detection probe capable of hybridizing with a tag sequence
  • 专利标题(中): 通过使用能够与标签序列杂交的检测探针来检测靶核酸的方法
  • 申请号: US10945245
    申请日: 2004-09-20
  • 公开(公告)号: US20050064488A1
    公开(公告)日: 2005-03-24
  • 发明人: Nam HuhSung-young JeongKui-hyun Kim
  • 申请人: Nam HuhSung-young JeongKui-hyun Kim
  • 优先权: KR2003-65218 20030919
  • 主分类号: C12P19/34
  • IPC分类号: C12P19/34 C12Q1/68
Method for detecting a target nucleic acid by using a detection probe capable of hybridizing with a tag sequence
摘要:
Provided is a method for detecting a target nucleic acid, comprising amplifying a target nucleic acid from a nucleic acid sample using a set of primers comprising at least one primer having a tag sequence at its 5′ end and a target binding sequence at its 3′ end; hybridizing the resulting amplified products with detection probes labeled with a label, the detection probes being capable of specifically binding to the tag sequence; hybridizing the hybridization products with capture probes immobilized on a microarray, the capture probes being capable of specifically binding to the target nucleic acid and not to the tag sequence; and determining the results of hybridization.
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