发明申请
US20050106719A1 Method of efficiently recombining orf encoded by cdna and template vector, trap vector and primer to be used therein
审中-公开
由cdna和模板载体有效重组或编码的方法，其中使用的捕获载体和引物
- 专利标题： Method of efficiently recombining orf encoded by cdna and template vector, trap vector and primer to be used therein
- 专利标题（中）： 由cdna和模板载体有效重组或编码的方法，其中使用的捕获载体和引物
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申请号： US10491192申请日： 2003-12-04
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公开(公告)号： US20050106719A1公开(公告)日： 2005-05-19
- 发明人: Takahiro Nagase , Daisuke Nakajima , Osamu Ohara
- 申请人： Takahiro Nagase , Daisuke Nakajima , Osamu Ohara
- 优先权： JP2002-357566 20021210
- 国际申请： PCT/JP03/15571 WO 20031204
- 主分类号： C12N15/10
- IPC分类号： C12N15/10 ; C12N15/64 ; C12N15/66 ; C12N15/85 ; C12N15/74
摘要：
The object of the present invention is to solve the problems in excising a DNA region (or gene) with conventional restriction enzymes, or in amplification of the DNA region by PCR. The present invention provides a method for precisely, quickly and simply cloning a target ORF, for example the target ORF encoding a protein, particularly the target ORF encoding a long-chain ORF comprising bases as many as several thousand bp in cDNA, and means such as various vectors used for the cloning method,
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