发明申请
US20050208501A1 Process and reagents for extraction of RNA from fractionated blood leukocytes
审中-公开
从分离的血液白细胞中提取RNA的方法和试剂
- 专利标题： Process and reagents for extraction of RNA from fractionated blood leukocytes
- 专利标题（中）： 从分离的血液白细胞中提取RNA的方法和试剂
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申请号： US10801982申请日： 2004-03-16
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公开(公告)号： US20050208501A1公开(公告)日： 2005-09-22
- 发明人: Marianna Goldrick
- 申请人： Marianna Goldrick
- 专利权人： AMBION, INC.
- 当前专利权人： AMBION, INC.
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; G01N33/543
摘要：
The current invention relates generally to methods for rapid fractionation of WBCs from whole blood using a proprietary leukocyte depletion filter, which concentrates and partially purifies the WBCs, and for stabilizing the RNA patterns in the fractionated cells. The invention further relates to methods of extracting the RNA from the fractionated cells and using such nucleic acids in any of a variety of molecular biology procedures including, for example, expression profiling.