An attachment and release apparatus for attaching a rotor to a centrifuge drive assembly and for attaching a cover to the rotor. The apparatus includes a knob, a cover wherein the knob has a plunger disposed therein. The apparatus also includes an adapter having detents and a drive spindle having detents. In addition, the apparatus has at least one adapter moveable element retained within a passage located on the knob and least one drive spindle moveable element retained within an additional passage located on the knob. The apparatus also includes a biasing element disposed around one end of the plunger.