发明申请
- 专利标题: Ascorbic acid production from yeast
- 专利标题(中): 酵母抗坏血酸生产
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申请号: US10606302申请日: 2003-06-25
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公开(公告)号: US20050260722A1公开(公告)日: 2005-11-24
- 发明人: Danilo Porro , Michael Sauer
- 申请人: Danilo Porro , Michael Sauer
- 专利权人: Biopolo S.C.a.R.L.
- 当前专利权人: Biopolo S.C.a.R.L.
- 主分类号: C12N15/09
- IPC分类号: C12N15/09 ; C12N1/19 ; C12N9/02 ; C12N9/04 ; C12N9/18 ; C12N15/52 ; C12N15/53 ; C12N15/55 ; C12P17/04 ; C12R1/645 ; C12N1/18
摘要:
Herein is disclosed a method of generating ascorbic acid from yeast. In one embodiment, the yeast is a Zygosaccharomyces spp. or a Kluyveromyces spp. growing in a medium comprising an ascorbic acid precursor. In a second embodiment the yeast is a recombinant yeast growing in a medium comprising an ascorbic acid precursor. Preferably the recombinant yeast is transformed with a coding region encoding an enzyme selected from L-galactose dehydrogenase (LGDH), L-galactono-1,4-lactone dehydrogenase (AGD), D-arabinose dehydrogenase (ARA), D-arabinono-1,4-lactone oxidase (ALO) or L-gulono-1,4-lactone oxidase (RGLO). The ascorbic acid precursor is preferably D-glucose, L-galactose, L-galactono-1,4-lactone, or L-gulono-1,4-lactone. In another preferred embodiment the ascorbic acid is accumulated in the medium at levels greater than background. Preferably, the yield of the conversion of the precursor to ascorbic acid is preferably at least about 35%.
公开/授权文献
- US07579171B2 Ascorbic acid production from yeast 公开/授权日:2009-08-25