发明申请
- 专利标题: Polypeptide
- 专利标题(中): 多肽
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申请号: US10886504申请日: 2004-07-07
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公开(公告)号: US20060008888A1公开(公告)日: 2006-01-12
- 发明人: Karsten Kragh , Bo Sorensen
- 申请人: Karsten Kragh , Bo Sorensen
- 主分类号: C12N9/00
- IPC分类号: C12N9/00 ; C07H21/04 ; C12P21/06
摘要:
We describe a food additive comprising a PS4 variant polypeptide, in which the PS4 variant polypeptide is derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 223 with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. The PS4 variant polypeptide may further comprise one or more further mutations at a position selected from the group consisting of: 121 and 161, preferably 121F, 121Y, 121W and 161A, more preferably G121F, G121Y, G121W and/or S161A, or the group consisting of: 134, 141, 157, 223, 307, 334, 33 and 34, preferably G134R, A141P, I157L, G223A, H307L, S334P, N33Y and D34N. In preferred embodiments, the PS4 variant polypeptide further comprises a mutation at position 87, preferably G87S, a mutation at position 178, preferably L178F, and/or a mutation at position 179, preferably A179T.