Invention Application
US20060013900A1 Use of betel leaf extract to induce IFN-gamma production from human peripheral blood T cells and as a Th1 type immunomodulator
审中-公开
使用槟榔叶提取物诱导来自人外周血T细胞和Th1型免疫调节剂的IFN-γ产生
- Patent Title: Use of betel leaf extract to induce IFN-gamma production from human peripheral blood T cells and as a Th1 type immunomodulator
- Patent Title (中): 使用槟榔叶提取物诱导来自人外周血T细胞和Th1型免疫调节剂的IFN-γ产生
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Application No.: US11222815Application Date: 2005-09-12
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Publication No.: US20060013900A1Publication Date: 2006-01-19
- Inventor: Santu Bandyopadhyay , Bikash Pal , Samir Bhattacharya , Mitali Ray , Keshab Roy
- Applicant: Santu Bandyopadhyay , Bikash Pal , Samir Bhattacharya , Mitali Ray , Keshab Roy
- Assignee: COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH ORGANISATION
- Current Assignee: COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH ORGANISATION
- Main IPC: A61K36/67
- IPC: A61K36/67
Abstract:
This invention relates to use of betel leaf extract to induce IFNγ from human peripheral blood mononuclear cells and as a Th1 type immuno modulator, wherein the said method comprises the steps of, preparing water extract of betel leaf, preparation of human peripheral blood mononuclear cells, incubation of hPBMC with betel leaf extract for a period of 18-48 hours, extraction of RNA for cytokine specific RT-PCR or for flow cytometry for the detection of intracellular cytokine protein, subjecting RNA for RT-PCR to obtain PCR products using IFNγ specific known primers, and enhancement of IFNγ as reflected by IFNγ specific band.
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