发明申请
- 专利标题： Real time PCR with the addition of pyrophosphatase
- 专利标题（中）： 实时PCR加入焦磷酸酶
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申请号： US11211481申请日： 2005-08-25
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公开(公告)号： US20060051796A1公开(公告)日： 2006-03-09
- 发明人: Inga Boell , Anja Degen , Dieter Heindl , Gregor Sagner
- 申请人： Inga Boell , Anja Degen , Dieter Heindl , Gregor Sagner
- 优先权： EPEP04021399.3 20040909
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12P19/34
摘要：
The present invention is directed to a method and a kit for amplifying and detecting a target nucleic acid, wherein the composition containing reagents to perform and monitor nucleic acid amplification in real time comprises at least a first hybridization probe labeled with a first fluorescent entity and a pyrophosphatase.
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