A pneumatic tire capable of eliminating the need of repairing puncture on the outside of a car and effectively preventing air leakage without accompanying an increase in weight and deterioration of riding comfortableness which occur in a conventional punctureless tire and a method of manufacturing the pneumatic tire, the pneumatic tire characterized in that a rubber-like thin-film formed of a latex dry thin-film in which 20 to 50 wt % rubber component is liquid isoprene rubber and having a breaking elongation of 900% or more and a tensile strength of 15 MPa or higher is disposed on the inner surface of the tire.