发明申请
- 专利标题: Methods for the production of cells and mammals with desired genetic modifications
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申请号: US10521634申请日: 2003-08-01
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公开(公告)号: US20060088898A1公开(公告)日: 2006-04-27
- 发明人: Brian Seed , Yi Yang
- 申请人: Brian Seed , Yi Yang
- 申请人地址: US MA Boston 02114
- 专利权人: The General Hospital Corporation
- 当前专利权人: The General Hospital Corporation
- 当前专利权人地址: US MA Boston 02114
- 国际申请: PCT/US03/24322 WO 20030801
- 主分类号: C12Q1/62
- IPC分类号: C12Q1/62 ; A01K67/027 ; C12N5/06
摘要:
The present invention features novel methods for generating cell lines and mammals with site-specific genetic modifications of interest. The methods involve homologous recombination between an artificial chromosome having a modification of interest and an endogenous chromosome of a cell. The resulting modified cells can be used in standard methods to generate genetically modified mammals. These mammals can be used in a variety of screening methods to identify compounds useful for the treatment or prevention of disease. Additionally, cells that have been modified to eliminate a mutation associated with a disease can be transplanted into patients for the treatment of a disease.