A non-pneumatic bicycle tire has a main tire body and at least one rim retaining member. The main tire body includes a rim engagement portion and a ground contact portion with a ground contact surface. The at least one rim retaining member is fixedly attached to the rim engagement portion. The at least one rim retaining member includes a pair of elastically deformable rim retaining portions extending laterally in opposite axial directions. Preferably, the main tire body has a plurality of the rim retaining members coupled thereto. In one embodiment, the rim retaining members are attached to the inner surface of the main tire body, while in another embodiment, the rim retaining members are embedded into the rim engagement portion of the main tire body.