This invention is related to a process for isolating and purifying lycopene, and more particularly to a process for large-scaled isolation and purification of the biosynthesized lycopene from bacterial cells via simple steps with high purity and recovery. The steps in the process of the present invention comprises: isolating a biomass from a fermented broth; treating the isolated biomass with alcohol; extracting the lycopene from the alcohol treated biomass with an organic solvent; removing the solid phase from the biomass/solvent extracting mixture; filtering the liquid-phase extract; crystallizing the lycopene by concentrating the liquid-phase extract; separating the dark red lycopene crystals; re-suspending the separated dark red lycopene crystals with acetone; and separating and drying the washed lycopene crystals. A biosynthesized lycopene purified by the process is also provided.