发明申请
- 专利标题： Methods to increase nucleotide signals by raman scattering
- 专利标题（中）： 通过拉曼散射增加核苷酸信号的方法
-
申请号： US11329693申请日： 2006-01-10
-
公开(公告)号： US20060166243A1公开(公告)日： 2006-07-27
- 发明人: Xing Su , Andrew Berlin , Selena Chan , Steven Kirch , Tac-Woong Koo , Gabi Neubauer , Valluri Rao , Narayanan Sundararajan , Mineo Yamakawa
- 申请人： Xing Su , Andrew Berlin , Selena Chan , Steven Kirch , Tac-Woong Koo , Gabi Neubauer , Valluri Rao , Narayanan Sundararajan , Mineo Yamakawa
- 专利权人： INTEL CORPORATION
- 当前专利权人： INTEL CORPORATION
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12P19/34
摘要：
The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid. In other embodiments, unlabeled nucleic acids are used. Exonuclease treatment of the nucleic acid results in the release of labeled or unlabeled nucleotides that are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides released from a nucleic acid by exonuclease treatment are covalently cross-linked to nanoparticles and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus for nucleic acid sequencing.
信息查询
