发明申请
- 专利标题： New expression system from rhodococcus
- 专利标题（中）： 来自红球菌的新表达系统
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申请号： US10537201申请日： 2003-12-02
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公开(公告)号： US20060172423A1公开(公告)日： 2006-08-03
- 发明人: Robert Van Der Geize , Gerda Hessels , Lubbert Dijkhuizen , Peter Van Der Meijden
- 申请人： Robert Van Der Geize , Gerda Hessels , Lubbert Dijkhuizen , Peter Van Der Meijden
- 优先权： EP02080054.6 20021203
- 国际申请： PCT/EP03/50928 WO 20031202
- 主分类号： C12N15/74
- IPC分类号： C12N15/74 ; C12N1/21 ; C07H21/04
摘要：
The present invention provides an isolated polynucleotide comprising the kstD promoter from Rhodococcus erythrypolis. The polynucleotide can very advantageously be used as a controllable transcription activator. Said controlling function can be provided by providing said isolated polynucleotide with a nucleotide sequence encoding a transcription regulator of said promoter. In the present invention, such a transcription regulator may be externally induced, such as by introduction of steroidal compounds. In an alternative embodiment of the present invention the isolated polynucleotide may comprise the kstR gene or a homologue or a functional part thereof as the transcription regulator of the kstD promoter.
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