A pixel structure electrically connected to a scan line and a data line, includes an active device, a first pixel electrode, a second pixel electrode, a capacitor coupling electrode and a charge releasing device. The active device is electrically connected to the scan line and the data line. The second pixel electrode is electrically isolated from the first pixel electrode. The capacitor coupling electrode is disposed under the second pixel electrode and electrically connected to the data line through the active device. The charge releasing device is electrically connected to the second pixel electrode. The above-described pixel structure is able to effectively solve the image sticking problem. In addition, further provides an active matrix substrate which is able to avoid the image sticking effect.