发明申请
- 专利标题： Method for monitoring reactions in real time
- 专利标题（中）： 实时监测反应的方法
-
申请号： US10562677申请日： 2004-06-29
-
公开(公告)号： US20060275854A1公开(公告)日： 2006-12-07
- 发明人: Yingfu Li , Razvan Nutiu
- 申请人： Yingfu Li , Razvan Nutiu
- 国际申请： PCT/CA04/00966 WO 20040629
- 主分类号： C12Q1/66
- IPC分类号： C12Q1/66 ; G06F19/00
摘要：
Methods for monitoring chemical reactions in real time are provided. The methods involve the use of a signaling aptamer that has a different affinity for the substrate and the product of the chemical reaction. The conversion from substrate to product is detected a change in signal, in particular a change in fluorescent signal. The methods of the present invention are also useful to measure enzyme activity and to screen for enzyme inhibitors.