Systems, methods and computer readable media for automatically inspecting a chemical array. At least one processor is adapted to receive a digitized image of the chemical array, and at least one of hardware, software and firmware are adapted to quantify at least one visual characteristic of a feature on the chemical array that contributes to uniformity of the visualization of the feature. Systems, methods and computer readable media are provided for automatically quantifying a visual characteristic of a chemical array. A digitized image of a chemical array having at least one feature is received, and at least one visual characteristic of a feature on the chemical array that contributes to uniformity of the visualization of the feature automatically quantified. A result based on the automatically quantification processing may be outputted to quantify at least one visual characteristic of a feature.