The present invention provides a lens holder comprising a third group frame that serves as a holding member which holds lenses, a guide shaft that supports the third group frame when the third group frame is moved along the optical axis of the lenses, a rear barrel part perpendicularly to which the guide shaft is locked, a first bearing member that is molded as an integral part of the rear barrel part in order to bear the end of the guide shaft distal to the rear barrel part, and a guide shaft presser that when the first bearing member bears the end of the guide shaft distal to the base, bears the end of the guide shaft proximal to the rear barrel part. Moreover, the present invention provides a lens barrel and an imaging apparatus that employ the lens holder. Consequently, the structure of a bearing that bears a shaft which supports the lens holding member when the lens holding member is moved along the optical axis of the lenses is downsized. Moreover, the bearing bears the shaft highly precisely.