发明申请
US20070054311A1 Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
审中-公开
通过DNA聚合过程产生的全基因组和整个转录组文库的扩增和分析
- 专利标题： Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
- 专利标题（中）： 通过DNA聚合过程产生的全基因组和整个转录组文库的扩增和分析
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申请号： US11593625申请日： 2006-11-06
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公开(公告)号： US20070054311A1公开(公告)日： 2007-03-08
- 发明人: Emmanuel Kamberov , Tong Sun , Eric Bruening , Jonathon Pinter , Irina Sleptsova , Takao Kurihara , Vladimir Makarov
- 申请人： Emmanuel Kamberov , Tong Sun , Eric Bruening , Jonathon Pinter , Irina Sleptsova , Takao Kurihara , Vladimir Makarov
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12P19/34
摘要：
The present invention regards a variety of methods and compositions for whole genome amplification and whole transcriptome amplification. In a particular aspect of the present invention, there is a method of amplifying a genome comprising a library generation step followed by a library amplification step. In specific embodiments, the library generating step utilizes specific primer mixtures and a DNA polymerase, wherein the specific primer mixtures are designed to eliminate ability to self-hybridize and/or hybridize to other primers within a mixture but efficiently and frequently prime nucleic acid templates.
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