An electrophoresis gel sample loader and method for making the same is disclosed. One embodiment includes the electrophoresis gel sample loader comprising a loader having a backing material and a plurality of porous sample holders mounted on the backing material. The porous sample holders retain a sample by absorption. In addition, the electrophoresis gel sample loader further includes a mounting layer comprising a plurality of dividers. At least one divider is disposed laterally to a porous sample holder.