An apparatus for controlling micro-fluid temperature is provided. The apparatus includes a chip, a fixed stand, a heat-absorption block, and an actuator. The chip has a reaction chamber for disposing a micro-fluid, and the temperature of the micro-fluid rises by heating the chip to reach the required reaction temperature. In the cooling-down operation, the heat-absorption block can contact the chip when pushed by the actuator, so as to dissipate the heat from the chip by way of heat conduction. Moreover, in the heating operation, the heat-conductive block is out of contact with the chip such that the micro-fluid in the chip is quickly heated, so as to reach the required reaction temperature of the micro-fluid rapidly.