发明申请
US20070087426A1 Novel transferase and amylase, process for producing the enzymes, use thereof, and gene coding for the same
审中-公开
新型转移酶和淀粉酶,用于生产酶的方法,其用途及其编码基因
- 专利标题: Novel transferase and amylase, process for producing the enzymes, use thereof, and gene coding for the same
- 专利标题(中): 新型转移酶和淀粉酶,用于生产酶的方法,其用途及其编码基因
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申请号: US11526632申请日: 2006-09-26
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公开(公告)号: US20070087426A1公开(公告)日: 2007-04-19
- 发明人: Masaru Kato , Yutaka Miura , Masako Kettoku , Akihiro Iwamatsu , Kazuo Kobayashi , Toshihiro Komeda
- 申请人: Masaru Kato , Yutaka Miura , Masako Kettoku , Akihiro Iwamatsu , Kazuo Kobayashi , Toshihiro Komeda
- 专利权人: Kirin Beer Kabushiki Kaisha
- 当前专利权人: Kirin Beer Kabushiki Kaisha
- 优先权: JP6-133354 19940615; JP6-194223 19940818; JP6-290394 19941031; JP6-286917 19941121; JP6-311185 19941121; JP7-120673 19950421
- 主分类号: C12N9/10
- IPC分类号: C12N9/10 ; C07H21/04
摘要:
The invention provides a novel transferase that acts on a saccharide, as a substrate, composed of at least three sugar units wherein at least three glucose residues on the reducing end are linked α-1,4 so as to transfer the α-1,4 lingages to a α-1, α-1 linkages; a process for producing the transferase; a gene coding for the same; and a process for producing an oligosaccharide by using the same. Also provided are a novel amylase that has a principal activity of acting on a saccharide, as a substrate, composed of at least three sugar units wherein at least three sugar units on the reducing end side are glucose units and the linkage between the first and the second glucose units is α-1, α-1 while the linkage between the second and the third glucose units is α-1,4 so as to liberate α, α-trehalose by hydrolyzing the α-1,4 linkage and another activity of hydrolyzing the α-1,4 linkage within the molecular chain of the substrate and that liberates disaccharides and/or monosaccharides as the principal final products; a process for producing the amylase; a gene coding for the same; and a process for producing α, α-trehalose by using a combination of the transferase and the amylase.