Methods of calibrating a lighting panel including a plurality of segments, each of said segments configured to emit a first color light and a second color light in response to pulse width modulation control signals having respective duty cycles include determining an average segment luminance for the lighting panel, determining a luminance variation of each segment to the average segment luminance, comparing the luminance variation of each segment to a threshold, and adjusting the duty cycle of at least one color of at least one segment to reduce the luminance variation in response to the luminance variation of a segment exceeding the threshold. Calibration systems are also disclosed.