A cavitation chamber separated into three volumes by a pair of gas-tight and liquid-tight seals, each seal formed by the combination of a rigid acoustic reflector and a flexible member, is provided. During chamber operation, only one of the three volumes contains cavitation fluid, the other two chamber volumes remaining devoid of cavitation fluid. The cavitation system also includes a cavitation fluid reservoir coupled to the cavitation chamber by a conduit, a valve allowing the cavitation chamber to be isolated from the cavitation fluid reservoir. A second conduit couples the two unfilled chamber volumes to a region above the liquid free surface within the cavitation fluid reservoir. A second valve allows the two unfilled chamber volumes to either be coupled to the cavitation fluid reservoir by the second conduit, or be coupled to a third conduit, the third conduit leading either to the ambient atmosphere or to a high pressure gas source. The cavitation system also includes at least one acoustic driver.