发明申请
US20070160995A1 Method for investigating cytosine methylation in dna by means of dna repair enzymes
失效
通过dna修复酶研究dna中胞嘧啶甲基化的方法
- 专利标题： Method for investigating cytosine methylation in dna by means of dna repair enzymes
- 专利标题（中）： 通过dna修复酶研究dna中胞嘧啶甲基化的方法
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申请号： US10585682申请日： 2005-01-10
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公开(公告)号： US20070160995A1公开(公告)日： 2007-07-12
- 发明人: Reimo Tetzner , Kurt Berlin , Jurgen Distler
- 申请人： Reimo Tetzner , Kurt Berlin , Jurgen Distler
- 申请人地址： DE Berlin 10178
- 专利权人： EPIGENOMICS AG
- 当前专利权人： EPIGENOMICS AG
- 当前专利权人地址： DE Berlin 10178
- 优先权： DE102004002257.7 20040109
- 国际申请： PCT/EP05/00231 WO 20050110
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12P19/34
摘要：
The following invention concerns a method for investigating cytosine methylation by means of DNA repair enzymes. Here, the DNA is first converted so that unmethylated cytosines are converted to uracil, while 5-methylcytosine remains unchanged. Then the DNA is hybridized to oligonucleotides, whereby hybrids will be formed with or without erroneous base pairings, in each case depending on the methylation status of the DNA. Following this, the erroneously paired hybrids will be cleaved by repair enzymes. Then the methylation status of the DNA can be determined in different ways. The method according to the invention is particularly suitable for the diagnosis and prognosis of cancer disorders and other diseases associated with a change of the methylation status as well as for predicting undesired drug effects.
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