发明申请
US20070178076A1 Porous membrane device that promotes the differentiation of monocytes into dendritic cells
审中-公开
促进单核细胞分化成树突状细胞的多孔膜装置
- 专利标题: Porous membrane device that promotes the differentiation of monocytes into dendritic cells
- 专利标题(中): 促进单核细胞分化成树突状细胞的多孔膜装置
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申请号: US11642926申请日: 2006-12-21
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公开(公告)号: US20070178076A1公开(公告)日: 2007-08-02
- 发明人: Donald Drake , David Moe , Conan Li , Heather Fahlenkamp , Guzman Sanchez-Schmitz , Russell Higbee , Robert Parkhill , William Warren
- 申请人: Donald Drake , David Moe , Conan Li , Heather Fahlenkamp , Guzman Sanchez-Schmitz , Russell Higbee , Robert Parkhill , William Warren
- 主分类号: A61K48/00
- IPC分类号: A61K48/00 ; C12M3/00 ; C12N5/08
摘要:
Dendritic cells (DCs) for research and clinical applications are typically derived from purified blood monocytes that are cultured in a cocktail of cytokines for a week or more. Because it has been suggested that these cytokine-derived DCs may be deficient in some important immunological functions and might not accurately represent antigen-presenting cell (APC) populations found under physiologic conditions, there is a need for methods that allow the generation of DCs in a more physiologically relevant manner. The present invention comprises a simple and reliable technique for generating large numbers of highly purified DCs, based on a single migration of blood monocytes through endothelial cells that are cultured in, for example, a Transwell® device. The resultant APCs, harvested from the lower Transwell® chamber, resemble other in vitro-generated DC populations in their expression of major histocompatibility (MHC) and costimulatory molecules, ability to phagocytose foreign antigens, and capacity to trigger antigen-specific T cell responses.
公开/授权文献
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