The present invention is a system to detect the presence of a queen bee, in a hive. The first system includes an RFID (radio frequency identification) tag (secured to the queen) and a RFID detection device secured adjacent the hive opening that sends an output that indicates the presence or absence of the queen bee. In a second embodiment, a bit of metal (thin foil) can be secured to the queen by means of an adhesive, and a metal detector can be positioned by the hive opening to detect the metal, and send an output that indicates the presence or absence of the queen bee. In a third embodiment, one or more infrared cameras can be positioned adjacent the hive entrance, coupled with detection scanning software that can detect the difference between a drone, a worker and a queen bee, and indicate the presence or absence of the queen bee.