An object of the invention is to provide a cartridge storing a plurality of sheet-shaped optical discs in a laminated manner in a state of being accommodated in trays, and a recording and reproducing apparatus of the optical disc. A cartridge stores a lot of trays in a laminated manner. The tray is formed by adhering a flat plate and a flat plate respectively having a smaller hole and a larger hole than a sheet-shaped optical disc, adhering a cover which can be opened and closed to the flat plate, and putting the optical disc in the large hole so as to be covered by the cover. The cartridge is moved up and down by a moving table, and the tray is taken out by being hooked by a hook mechanism. At this time, the cover is peeled out by a peeling claw. The optical disc is fixed to a spindle motor to which a glass disc is attached. The optical disc is rotated while being sucked to the glass disc by a fluid force, and is recorded and reproduced by a recording and reproducing portion. The recording and reproducing apparatus is structured such that the optical disc is turned back to the tray and is compressed to the cartridge by the hook mechanism while being covered by the cover.