发明申请
US20070212710A1 COMPETITIVE HYBRIDIZATION OF DNA PROBES AND METHOD OF USING THE SAME
审中-公开
DNA探针的竞争性杂交及其使用方法
- 专利标题: COMPETITIVE HYBRIDIZATION OF DNA PROBES AND METHOD OF USING THE SAME
- 专利标题(中): DNA探针的竞争性杂交及其使用方法
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申请号: US11619143申请日: 2007-01-02
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公开(公告)号: US20070212710A1公开(公告)日: 2007-09-13
- 发明人: Michael Mittmann
- 申请人: Michael Mittmann
- 申请人地址: US CA Santa Clara 95051
- 专利权人: Affymetrix, INC.
- 当前专利权人: Affymetrix, INC.
- 当前专利权人地址: US CA Santa Clara 95051
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C07H21/04
摘要:
Methods are presented, in accordance for the present invention, for determining the length of a target probe. The methods have the steps of designing a first hybridization probe having a nucleic acid sequence, a portion of which overlaps with the nucleic acid sequence of a second hybridization probe, designing a second hybridization probe having a nucleic acid sequence, a portion of which overlaps with the nucleic acid sequence of the first hybridization probe, designing a target probe having the nucleic acid sequences of both the first and second hybridization probe and affixing the target probe to a solid support, labeling one of the first and second hybridization probes, but not both, and contacting simultaneously the first and second probes to the target probe, and detecting and quantifying the signal intensity ration between the labeled and non-labeled probes, whereby said ration indicating whether the target probe synthesis has reached full length.
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