A method of operating a front opening shipping box is provided. The operating method comprises providing a front opening shipping box. The front opening shipping box includes at least a container body, at least an air extraction pipeline and at least a gas inlet pipeline. The container body has at least two openings. The air extraction pipeline is disposed on the surface of the container body and linked to the container body through one of the two openings. The gas inlet pipeline is also disposed on the surface of the container body and linked to the container body through the other opening. Then, a number of wafers is loaded inside the container body. Next, through the air extraction pipeline and the gas inlet pipeline, air is pumped out from the inside of the container box while the gas is injected into the container body simultaneously.