A validation system includes: a source agent for storing a plurality of test patterns; a drain agent for performing a validation operation according to a test result; and a device under test (DUT). The device under test includes: a first interface electrically connected to the source agent for communicating with the source agent and receiving the test patterns outputted from the source agent; a target system electrically connected to the first interface for processing the test patterns to generate a plurality of test results; and a second interface electrically connected to the target system and the drain agent for communicating with the drain agent and transferring the plurality of test results to the drain agent.