发明申请
- 专利标题: Double-Stranded Ribonucleic Acid with Increased Effectiveness in an Organism
- 专利标题(中): 双链核糖核酸增加有机体的有效性
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申请号: US10560336申请日: 2004-06-14
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公开(公告)号: US20070275465A1公开(公告)日: 2007-11-29
- 发明人: Claudia Woppmann , Hans-Peter Vornlocher , Philipp Hadwiger , Matthias John , Stefan Limmer
- 申请人: Claudia Woppmann , Hans-Peter Vornlocher , Philipp Hadwiger , Matthias John , Stefan Limmer
- 申请人地址: US MA Cambridge 02142
- 专利权人: Alnylam Pharmaceuticals
- 当前专利权人: Alnylam Pharmaceuticals
- 当前专利权人地址: US MA Cambridge 02142
- 优先权: EP03013296.3 20030613; EP04002374.9 20040203
- 国际申请: PCT/US04/18848 WO 20040614
- 主分类号: C07H21/02
- IPC分类号: C07H21/02
摘要:
The invention concerns a method for the targeted selection of a double-stranded ribonucleic acid (dsRNA) consisting of two single strands that exhibits increased effectiveness in inhibiting the expression of a target gene by means of RNA interference, whereby the sequences of the single strands of the dsRNA are selected in such a way that on both ends of the dsRNA the last complementary nucleotide pair is a G-C, or at least two of the last four complementary nucleotide pairs are G-C pairs; whereby the dsRNA exhibits a single-stranded overhang consisting of 1 to 4 unpaired nucleotides at the first end, and no overhang at the second end; whereby the unpaired nucleotide of the single-stranded overhang that is directly adjacent to the last complementary nucleotide pair contains a purine base.