发明申请
- 专利标题： 3'-End Nucleoside Unit Comprising Phosphoramidite
- 专利标题（中）： 包含亚磷酰胺的3'-末端核苷单位
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申请号： US10590268申请日： 2005-02-10
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公开(公告)号： US20080039620A1公开(公告)日： 2008-02-14
- 发明人: Mitsuo Sekine , Kohji Seio , Akihiro Ohkubo
- 申请人： Mitsuo Sekine , Kohji Seio , Akihiro Ohkubo
- 申请人地址： JP Kawaguchi-shi, Saitama 332-0012
- 专利权人： Japan Science and Technology Agency
- 当前专利权人： Japan Science and Technology Agency
- 当前专利权人地址： JP Kawaguchi-shi, Saitama 332-0012
- 优先权： JP2004-049312 20040225
- 国际申请： PCT/JP05/02058 WO 20050210
- 主分类号： C07H21/04
- IPC分类号： C07H21/04 ; C08F8/40
摘要：
The purpose of the present invention is therefore to provide a method for binding a 3′-end nucleoside unit comprising any base to a hydroxyl group on a solid-phase support under completely the same condition as in DNA chain elongation reaction. The present invention relates to a 3′-end nucleoside unit comprising phosphoramidite that is a compound represented by the following formula: (N)—O—(R1)Si(R2)-(C6H4)—(CH2)n-O—P(OR3)N(R4)(R5) (I) wherein (N) represents any nucleoside or its derivative, each of R1, R2, R4 and R5 is an alkyl or aryl group, R3 is a phosphate-protecting group, and n is an integer of from 1 to 5; a solid-phase support having said 3′-end nucleoside unit; and a method for the synthesis of a nucleic acid oligomer with the use of said solid-phase support.
公开/授权文献
- US07943758B2 3′-end nucleoside unit comprising phosphoramidite 公开/授权日：2011-05-17
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