发明申请
US20080088823A1 Optical Train and Method for TIRF Single Molecule Detection and Analysis
审中-公开
光学列车及TIRF单分子检测与分析方法
- 专利标题： Optical Train and Method for TIRF Single Molecule Detection and Analysis
- 专利标题（中）： 光学列车及TIRF单分子检测与分析方法
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申请号： US11849088申请日： 2007-08-31
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公开(公告)号： US20080088823A1公开(公告)日： 2008-04-17
- 发明人: Timothy Harris , Philip Buzby , Mirna Jarosz , Jaime Gill , Howard Weiss , Stanley Lapidus
- 申请人： Timothy Harris , Philip Buzby , Mirna Jarosz , Jaime Gill , Howard Weiss , Stanley Lapidus
- 申请人地址： US MA Cambridge 02139
- 专利权人： HELICOS BIOSCIENCES CORPORATION
- 当前专利权人： HELICOS BIOSCIENCES CORPORATION
- 当前专利权人地址： US MA Cambridge 02139
- 主分类号： G01J3/00
- IPC分类号： G01J3/00 ; G01N21/00 ; G01N21/55
摘要：
In one aspect the invention relates to an apparatus for analyzing the presence of a single molecule using total internal reflection. In one embodiment an apparatus for single molecule analysis includes a support having a sample located thereon; two sources of light at distinct wavelengths, a collimator for directing the light onto the sample through a total internal reflection objective; a receiver for receiving a fluorescent emission produced by a single molecule in the sample in response to the light; and a detector for detecting each of the wavelengths in the fluorescent emission. In another embodiment the apparatus further comprises a focusing laser for maintaining focus of the objective on the sample.