发明申请
- 专利标题： Rho Antibody And Tag To Purify Cell Surface Proteins
- 专利标题（中）： Rho抗体和标签来纯化细胞表面蛋白
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申请号： US10573610申请日： 2004-09-27
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公开(公告)号： US20080097084A1公开(公告)日： 2008-04-24
- 发明人: Michael J. Mendez , Craig Behnke , Craig Hokanson
- 申请人： Michael J. Mendez , Craig Behnke , Craig Hokanson
- 国际申请： PCT/US04/31831 WO 20040927
- 主分类号： C07K1/107
- IPC分类号： C07K1/107 ; C07K1/14 ; C07K14/00
摘要：
A process for the expression and purification of membrane associated proteins is provided. A process for the expression and purification of membrane proteins such as G-protein coupled receptors is provided. A hybrid polypeptide is constructed from a protein of interest and a peptide tag from the amino-terminus extracellular domain of rhodopsin. A method for purifying the hybrid protein using the rhodopsin tag is provided. Methods of detecting the expressed hybrid are also provided.
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