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发明申请
US20080138845A1 Method of De Novo Sequencing of Peptide Using the Maldi Mass Spectrometry, Method and Apparatus for Preparing Sample for Maldi Mass Spectrometry 有权
使用Maldi质谱测定肽的新方法，Maldi质谱法制备样品的方法和装置

Method of De Novo Sequencing of Peptide Using the Maldi Mass Spectrometry, Method and Apparatus for Preparing Sample for Maldi Mass Spectrometry
摘要：
The present invention relates to a method of peptide sequencing by MALDI (matrix-assisted laser desorption ionization) tandem mass spectrometry, which comprises the steps of chemically modifying a sample peptide with at least one chemical modification method selected from the group consisting of guanidination and esterification in order to change the ionization status of the peptide and performing mass spectrometry using a MALDI tandem mass spectrometer and programmed MALDI tandem mass spectrometry. The peptide sequencing method of the present invention is advantageous in that detection sensitivity of the peptide improves significantly and various daughter ions are detected uniformly, thereby enabling perfect de novo sequencing with tandem mass spectrometry only, without database search.
摘要（中）：

本发明涉及通过MALDI（基质辅助激光解吸电离）串联质谱法进行肽测序的方法，其包括用至少一种选自胍基和酯化的化学修饰方法化学修饰样品肽的步骤 以改变肽的电离状态，并使用MALDI串联质谱仪和程序化的MALDI串联质谱进行质谱分析。 本发明的肽测序方法的优点在于，肽的检测灵敏度显着提高，并且均匀地检测各种子离子，从而仅通过串联质谱法即可完成从头测序，无需数据库检索。

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