An apparatus for performing live cell analysis includes a stage housing and a chamber assembly. The stage housing includes a cover having a bottom surface. The chamber assembly is movably disposed below and movable relative to the cover of the stage housing and can removably receive a specimen plate holding live cells. The chamber assembly includes a chamber housing having a perimeter wall with an interior surface and an exterior surface extending from an upper end to a spaced apart lower end. The perimeter wall bounds a compartment that passes all the way through the chamber housing from the upper end to the lower end. At least one gas outlet port is formed on the chamber housing so as to be in communication with the compartment of the chamber housing to allow gas to enter the compartment. A light can be mounted to the cover to facilitate high content screening of the live cells using a microscope in bright field mode.