A non-aqueous electrolyte secondary battery comprising a negative electrode having a current collector provided with convex portions and concave portions on both surfaces, a first columnar body formed in a tilting orientation on the convex portion on one of the surfaces of the current collector, and a second columnar body formed in a tilting orientation on the convex portion on the other surface, a positive electrode having a positive electrode current collector provided with a positive electrode mixture layer containing a positive electrode active material capable of reversibly inserting and extracting lithium ions on both surfaces, and a separator interposed between the positive electrode and the negative electrode in a confronting manner, wherein the first columnar body and the second columnar body on the negative electrode are tilted to an orthogonal direction of a winding direction of the current collector.