发明申请
- 专利标题: Screening using polarization anisotropy in FRET emissions
- 专利标题(中): 在FRET排放中使用偏振各向异性进行筛选
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申请号: US12069724申请日: 2008-02-11
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公开(公告)号: US20080206888A1公开(公告)日: 2008-08-28
- 发明人: Steven C. Miller , Paul B. Comita , Christopher B. Shumate , Evan F. Cromwell
- 申请人: Steven C. Miller , Paul B. Comita , Christopher B. Shumate , Evan F. Cromwell
- 专利权人: Blueshift Biotechnologies, Inc.
- 当前专利权人: Blueshift Biotechnologies, Inc.
- 主分类号: G01N33/566
- IPC分类号: G01N33/566 ; G01N21/76
摘要:
Methods and apparatus are described for detecting specific binding between first and second chemical entities. The first chemical entity in association with a first fluorophore is immobilized. The second chemical entity is allowed to bind with the immobilized first chemical entity. The second chemical entity is or becomes coupled to a second fluorophore, which forms a FRET pair with the first fluorophore. The bound chemical entities are exposed to radiation at an excitation frequency for either the first or the second fluorophore, and polarization anisotropy of a FRET fluorescent signal from the bound chemical entities is measured to detect specific binding between the first and second chemical entities. Techniques are also disclosed for detecting whether a FRET interaction is occurring between a first chemical entity including a donor fluorophore and a second chemical entity including an acceptor fluorophore, using simultaneous anisotropy measurements at the wavelengths of the donor and acceptor fluorophores.
公开/授权文献
- US07674588B2 Screening using polarization anisotropy in FRET emissions 公开/授权日:2010-03-09