A PCI Express interface card includes at least two different sized PCI Express connecting interfaces (40), at least two PCI Express buses (50) corresponding to the at least two PCI Express connecting interfaces, a controlling module (10), a switching module (20), and an indicating module (30). The at least two PCI Express connecting interfaces are used to connect with at least two corresponding PCI Express slots on a motherboard (60) to be tested. The controlling module is connected to the at least two PCI Express connecting interfaces via the at least two corresponding PCI Express buses, and communicates with the at least two PCI Express connecting interfaces. The switching module switches the controlling module to corresponding modes according to which of the PCI Express slots is connected with a corresponding PCI Express connecting interface. The indicating module is electrically connected with the controlling module, and indicates whether a test is passed.