发明申请
- 专利标题: METHOD OF MEASURING HUMAN CYP3A INDUCIBILITY
- 专利标题(中): 测量人CYP3A诱导的方法
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申请号: US12180822申请日: 2008-07-28
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公开(公告)号: US20090029351A1公开(公告)日: 2009-01-29
- 发明人: Yasushi YAMAZOE , Kiyoshi Nagata
- 申请人: Yasushi YAMAZOE , Kiyoshi Nagata
- 申请人地址: JP Sendai-shi JP Tokyo
- 专利权人: Yasushi YAMAZOE,DAIICHI PURE CHEMICALS CO., LTD.
- 当前专利权人: Yasushi YAMAZOE,DAIICHI PURE CHEMICALS CO., LTD.
- 当前专利权人地址: JP Sendai-shi JP Tokyo
- 优先权: JP2002.112364 20020415
- 主分类号: C12Q1/70
- IPC分类号: C12Q1/70 ; C12Q1/68
摘要:
A method for measuring human CYP3A inducibility upon administration of a test drug, characterized in that a non-human animal to which a test drug is administered or a population of human cells cultured in a medium containing a test drug is infected with viruses (A) and (B); virus (A) being an adenovirus which is used as a vector and engineered by incorporating thereto a detectable reporter gene and at least 3 human PXR binding regions falling within an untranslated region of a human CYP3A gene, and virus (B) being an adenovirus which is used as a vector and engineered by incorporating thereto a human PXR cDNA; and subsequently expression level of the reporter gene is determined in the non-human animal or the cultured human cells.The present invention ensures convenient and accurate evaluation of human CYP3A inducibility upon administration of a test drug to a human subject, providing accurate evaluation in terms of the efficacy of the test drug, occurrence of side effects, disappearance of the drug effect, etc.
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