A sealed and decontaminated fiber optic detection apparatus includes an optics portion with individual chambers. Each chamber housing optical and electro-optical components. A manifold accommodates fibers, with each of the fibers being in optical communication with the optical and electro-optical components of a corresponding chamber. The apparatus also includes a sample holder that holds a sample to be tested and a mounting device provided between the sample holder and the manifold. The mounting device and the manifold form a sealed fiber optic interface between the sample holder and the optics portion.